Wednesday, April 27, 2011
Success.
All hyped up and ready to finally do my mini-preps correctly, I set everything up and began. As I was doing one step, Jennifer noticed that I didn't mix up my cells well enough. She told me that not fully mixing them up mean that I didn't even open up the DNA so the parts that I didn't want couldn't be cut from the parts of the DNA that I did want. Yet again this proved to me that attention to the detail within the protocols is really the most important part of working in a lab. Sure enough when I went to do my nano-drops all of the DNA samples came out above 200 ng/ul! I was relieved that I had finally done it right and that we could move on to the next and near final stage of our experiment. I'm not exactly sure yet what I'll be doing tomorrow but I don't think it will be anything big since I'm leaving Friday for Florida. It's kind of inconvenient that I have to leave Friday, and I wont even be back into the lab until Thursday, almost a full week! It really delays the experiment and everything that I'm trying to get done, but I know in the end I'll have a blast in Florida and have lots of fun stories to tell everyone when I get back!
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