Tuesday, April 26, 2011

Not Again!

So I went in this morning all ready to do my mini-preps and do them successfully this time! I went through each step carefully and slowly, taking time to make sure that I did each step just as it was stated. After taking an abnormally long time to complete my mini-preps I went upstairs to the nano-drop to see if I had a sufficient amount of DNA in each of my tubes. On average, 200ng/ul of DNA is around the lowest you want. I nano-dropped my first tube; 13.8 ng/ul. This isn't looking too good I thought to myself... As I nano-dropped the rest I came to find that all of them were just as low, with the exception of two that were around 180 ng/ul. I was really disappointed in myself and almost scared to go tell Jennifer that I had failed at my mini-preps yet again. I could tell she was a little disappointed as well but didn't seem to be mad about it. I was beginning to get really down on myself, maybe this isn't the right job for me? On the car ride home I though about it a lot; I'm only in high-school! Most people don't start doing this until they're have at least one or two years of a college education under their belt, sometimes even more than that! It made me feel a lot better and I went home ready to finally get things right the next day.

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