Transformation

When I came in today I was happy to find that I actually had cells on my plates! I had used different cells from the last time I plated so I assume thats why it worked out this time. I had to calculate transformation efficiencies for both the old cells I used and the new ones and it turns out the efficiency on the old cells that I used was a little bit lower than it should be, maybe that could have also had an effect on my colonies and prevented them from growing. The day altogether was a very long one, I worked from 9AM to 5PM. Surprisingly it didn't even seem as though it was eight hours. With only one hour long lunch break, the day still seemed to fly by. I'm hoping that's a good sign and shows me that I'm actually truly enjoying what I'm doing. Today when I was leaving I got a little sad that my time here is almost over. Maybe I'll be able to get an internship and come back this summer?